Bioactive Nanomaterials from Self-assembled & Polymerized Peptide-Amphiphiles: Towards Cell-based Microsystems (Oberflächenchemie und -physik von Mikrosystemen) - Shroff, Kamlesh
Netto: 16,26 €17,4€
inkl. MwSt. zzgl. Versand
Bearbeitungszeit: 3 Werktage
Sofort lieferbar (auf Lager)
1x Stück verfügbar
Artikelzustand Mangelware (nachgebunden):
- Stark gebrauchter Zustand / Mangelware
- Buchrücken fehlt und wurde maschinell nachgebunden
- Seiten können fehlen, weil die Prüfung aller Seiten zu zeitaufwendig ist
- Coverseiten können vom Text abgeschnitten sein
- Vereinzelte Seiten können lose sein
- Blattübergänge können Unterschiede aufweisen
- Es handelt sich um Jahrzehnte alte Bücher, die nicht für Allergiker oder anspruchsvolle Kunden geeignet sind
Buchzusammenfassung:
Preface Interesting novel hybrid microsystems are expected, if living cells are combined with artificial materials used in MEMS devices. Possible applications progress from novel cell-based biosensors to drug development and biocompatible surface coatings. The keyparameter that determines such applications is the understanding and manipulation of cellular adhesion on artificial materials (i.e. surfaces). To this, the pursuit for novel bioengineered materials has drawn increasing attention towards the development of synthetic functionalized materials that interact with living systems in a preferred way. For example, engineering bioactive ligands (e.g. small peptide-motifs) into artificial materials is one of the most promising approaches to design bioactive surfaces that are capable of mimicking the natural surrounding of living cells, i.e. the extracellular matrix. An interesting approach is the attachment of functional cell-adhesion ligands, like the well-known “RGD”-peptide to small lipid molecules, thereby forming so-called “peptide-amphiphiles”. These peptide amphiphiles can self-assemble into a variety of 2D and 3D architectures e.g. lipid monolayers and vesicles. The advantage of using such small bioactive building blocks is the high level of control over important parameters such as the ligand surface-presentation (i.e. manipulating the concentration and distance of the bioactive ligand at the surface). In this work, we report on the synthesis and use of polymerizable peptide-amphiphiles, i.e. molecular building blocks that consist of a polymerizable fatty acid, which is linked to a cell-adhesion promoting peptide. The peptide-amphiphile is further used to generate solid supported polymerized monolayers as well as vesicles in solution. The physico-chemical properties of these materials are characterized, as well as the biological activity has been investigated by the use of model cell-lines. We also present a design principle for the development of cell-based microarrays using surface-immobilized polymerized vesicles that expose the molecular recognition site at the outer surface, and hence spatially control the cell-attachment on solid surfaces.
